感染症学研究室

玉井 栄治　業績

 

論文

  1. 1)Structural and biochemical characterization of Clostridium perfringens pili protein B collagen-binding domains.

    Tamai E, Yamada M, Ishida T, Arimura N, Matsunami R, Sekiya H, Kamitori S.

    FEBS Lett. 2023 Apr 18. doi: 10.1002/1873-3468.14626.


  1. 2)Biochemical Characterizations of the Putative Endolysin Ecd09610 Catalytic Domain from Clostridioides difficile.

     Sekiya H, Yamaji H, Yoshida A, Matsunami R, Kamitori S, Tamai E.

     Antibiotics (Basel). 2022 Aug 20;11(8):1131. doi: 10.3390/antibiotics11081131.


  1. 3) Structural and biochemical characterization of the Clostridium perfringens-specific Zn2+-dependent amidase endolysin, Psa, catalytic domain.

    Hiroshi Sekiya, Shigehiro Kamitori, Hirofumi Nariya, Risa Matsunami, Eiji Tamai

    Biochem Biophys Res Commun. 2021 Aug 30;576:66-72


  1. 4) X-ray structures of Clostridium perfringens sortase C with C-terminal cell wall sorting motif of LPST demonstrate role of subsite for substrate-binding and structural variations of catalytic site.

    Eiji Tamai, Hiroshi Sekiya, Hirofumi Nariya, Seiichi Katayama, Shigehiro Kamitori

    Biochem Biophys Res Commun. 2021 May 21;554:138-144.


  1. 5)A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm

    Hiroshi Sekiya, Maho Okada, Eiji Tamai, Toshi Shimamoto, Tadashi Shimamoto,

    Hirofumi Nariya

    Antibiotics (Basel) 2021 Mar; 10(3): 245.


  1. 6)Structural and biochemical characterizations of the novel autolysin Acd24020 from Clostridioides difficile and its full‐function catalytic domain as a lytic enzyme 

    Hiroshi Sekiya, Eiji Tamai, Jurina Kawasaki, Kaho Murakami, Shigehiro Kamitori

    Mol Microbiol. 2021 Apr;115(4):684-698.


  1. 7) Benzoxazole-Based Metal Complexes to Reverse Multidrug Resistance in Bacteria

     Annamária Kincses, Stefánia Szabó, Bálint Rácz, Nikoletta Szemerédi, Genki Watanabe,

     Ryosuke Saijo, Hiroshi Sekiya, Eiji Tamai, Joseph Molnár, Masami Kawase,

     Gabriella Spengler

    Antibiotics (Basel) 2020 Oct; 9(10): 649


  1. 8)Structures of major pilins in Clostridium perfringens demonstrate dynamic conformational change.

  　Tamai E, Katayama S, Sekiya H, Nariya H, Kamitori S.

　 Acta Crystallogr D Struct Biol. 2019 Aug 1;75(Pt 8):718-732.


  1. 9)Benzoxazole-based Zn(II) and Cu(II) Complexes Overcome Multidrug-resistance in Cancer.

    Spengler G, Kincses A, Rácz B, Varga B, Watanabe G, Saijo R, Sekiya H, Tamai E, Maki J,

    Molnár J, Kawase M.

    Anticancer Res. 2018 Nov;38(11):6181-6187.


  1. 10)Synthesis and Antimicrobial Activity of 2-Trifluoroacetonylbenzoxazole Ligands and Their

    Metal Complexes.

    Watanabe G, Sekiya H, Tamai E, Saijo R, Uno H, Mori S, Tanaka T, Maki J, Kawase M.

    Chem Pharm Bull (Tokyo). 2018;66(7):732-740.


  1. 11) Expression of glyceraldehyde-3-phosphate dehydrogenase on the surface of Clostridium perfringens cells.

    Matsunaga N, Shimizu H, Fujimoto K, Watanabe K, Yamasaki T, Hatano N, Tamai E, 

    Katayama S, Hitsumoto Y.

    Anaerobe. 2018 Jun;51:124-130.


  1. 12) X-ray structure of Clostridium perfringens sortase B cysteine transpeptidase.

     Tamai E, Sekiya H, Maki J, Nariya H, Yoshida H, Kamitori S.

     Biochem Biophys Res Commun. 2017 Nov 25;493(3):1267-1272.


  1. 13) Identification of Characteristic Phenolic Constituents in Mousouchiku Extract Used as Food Additives.

    Yoshimura M, Ochi K, Sekiya H, Tamai E, Maki J, Tada A, Sugimoto N, Akiyama H,

    Amakura Y.

    Chem Pharm Bull (Tokyo). 2017;65(9):878-882.


  1. 14) A Novel Methodology for Synthesis of 1,5,6-Trisubstituted 2(1H)-Pyrazinones of

     Biological Interest.

     Saijo R, Sekiya H, Tamai E, Kurihara KI, Maki J, Sakagami H, Kawase M.

     Chem Pharm Bull (Tokyo). 2017;65(4):365-372.


  1. 15)Structural and biochemical characterization of the Clostridium perfringens autolysin catalytic domain.

    Tamai E, Sekiya H, Goda E, Makihata N, Maki J, Yoshida H, Kamitori S.

    FEBS Lett. 2017 Jan;591(1):231-239.


  1. 16)Identification of Characteristic Phenolic Constituents in Mousouchiku Extract Used as Food Additives.

    Yoshimura M, Ochi K, Sekiya H, Tamai E, Maki J, Tada A, Sugimoto N, Akiyama H,        

    Amakura Y.

    Chem Pharm Bull (Tokyo). 2017;65(9):878-882.


  1. 17)A Novel Methodology for Synthesis of 1,5,6-Trisubstituted 2(1H)-Pyrazinones of Biological Interest.

    Saijo R, Sekiya H, Tamai E, Kurihara KI, Maki J, Sakagami H, Kawase M.

    Chem Pharm Bull (Tokyo). 2017;65(4):365-372


  1. 18)X-ray structure of a novel endolysin encoded by episomal phage phiSM101 of Clostridium perfringens.

    Tamai E, Yoshida H, Sekiya H, Nariya H, Miyata S, Okabe A, Kuwahara T, Maki J,

    Kamitori S.

    Mol. Microbiol. 2014 Apr;92(2):326-337.


  1. 19) Identification and characterization of a putative endolysin encoded by episomal phage phiSM101 of Clostridium perfringens.

    Nariya H, Miyata S, Tamai E, Sekiya H, Maki J, Okabe A.

    Appl. Microbiol. Biotechnol. 2011 Jun;90(6):1973-1979.


  1. 20) Development and Application of a Method for Counterselectable In-Frame Deletion in Clostridium perfringens.

    Nariya H, Miyata S, Suzuki M, Tamai E Okabe A.

    Appl. Environ. Microbiol. 2011 Feb;77(4):1375-1382.


  1. 21) Identification and characterization of a Vibrio mimicus gene encoding the heme/hemoglobin receptor.

    Tanabe T, Funahashi T, Moon YH, Tamai E, Yamamoto S.

    Microbiol. Immunol. 2010 Oct;54(10):606-617.


  1. 22) High-level production and purification of clostripain expressed in a virulence-attenuated strain of Clostridium perfringens.

    Tanaka H, Nariya H, Suzuki M, Houchi H, Tamai E, Miyata S, Okabe A.

   Protein Expr. Purif. 2011 Mar;76(1):83-89.


  1. 23) Infections with gastrointestinal parasitic helminths indigenous to Japan and their treatment historically studied in an attempt to control the diseases in countries where they are still rampant: (1) the Jomon to Edo periods.

    Maki J, Sakagami H, Kuwada M, Caceres A, Sekiya H, Tamai E.

    Yakushigaku Zasshi. 2009;44(1):18-23.　


  1. 24) High-level expression of his-tagged clostridial collagenase in Clostridium perfringens.

    Tamai E, Miyata S, Tanaka H, Nariya H, Suzuki M, Matsushita O, Hatano N, Okabe A.

    Appl. Microbiol. Biotechnol. 2008 Sep;80(4):627-635


  1. 25) Construction of the curriculum and lectures for the subject, "History of pharmacy" in Matsuyama University School of Pharmacy.

    Maki J, Sakagami H, Kuwada M, Sekiya H, Tamai E.

    Yakushigaku Zasshi. 2008;43(2):181-184.


  1. 26)Construction and characterization of a clostripain-like protease-deficient mutant of Clostridium perfringens as a strain for clostridial gene expression.

    Tanaka H, Tamai E, Miyata S, Taniguchi Y, Nariya H, Hatano N, Houchi H, Okabe A.

    Appl. Microbiol. Biotechnol. 2008 Jan;77(5):1063-1071.


  1. 27) [Optimum preparation of levocarnitine chloride solution in the hospital pharmacy].

    Tanaka H, Asakura M, Doi C, Fukuoka N, Tamai E, Miyata S, Matsushita O, Okabe A,    

    Negayama K, Houchi H.

    Yakugaku Zasshi. 2006 Sep;126(9):805-809. Japanese.


  1. 28) Changes in ganglioside content affect the binding of Clostridium perfringens epsilon-toxin to detergent-resistant membranes of Madin-Darby canine kidney cells.

    Shimamoto S, Tamai E, Matsushita O, Minami J, Okabe A, Miyata S.

    Microbiol. Immunol. 2005;49(3):245-253.


  1. 29) Genomic studies with Escherichia coli MelR protein: applications of chromatin immunoprecipitation and microarrays.

    Grainger DC, Overton TW, Reppas N, Wade JT, Tamai E, Hobman JL, Constantinidou C,

    Struhl K, Church G, Busby SJ.

    J. Bacteriol. 2004 Oct;186(20):6938-6943.


  1. 30) High-level expression of clostridial sialidase using a ferredoxin gene promoter-based plasmid.

    Takamizawa A, Miyata S, Matsushita O, Kaji M, Taniguchi Y, Tamai E, Shimamoto S,

    Okabe A.

   Protein Expr. Purif. 2004 Jul;36(1):70-75.


  1. 31) A novel type of DNA curvature present in a Clostridium perfringens ferredoxin gene: characterization and role in gene expression.

    Kaji M, Matsushita O, Tamai E, Miyata S, Taniguchi Y, Shimamoto S, Katayama S,

    Morita S, Okabe A.

    Microbiology. 2003 Nov;149(Pt 11):3083-3091.


  1. 32) Accumulation of Clostridium perfringens epsilon-toxin in the mouse kidney and its possible biological significance.

    Tamai E, Ishida T, Miyata S, Matsushita O, Suda H, Kobayashi S, Sonobe H, Okabe A.

   Infect Immun. 2003 Sep;71(9):5371-5375.


  1. 33) Clostridium perfringens epsilon-toxin forms a heptameric pore within the detergent-insoluble microdomains of Madin-Darby canine kidney cells and rat synaptosomes.

    Miyata S, Minami J, Tamai E, Matsushita O, Shimamoto S, Okabe A.

    J. Biol Chem. 2002 Oct 18;277(42):39463-39468.


  1. 34) Purification, reconstitution, and characterization of Na(+)/serine symporter, SstT, of Escherichia coli.

    Kim YM, Ogawa W, Tamai E, Kuroda T, Mizushima T, Tsuchiya T.

    J. Biochem. 2002 Jul;132(1):71-76.


  1. 35) Phased A-tracts bind to the alpha subunit of RNA polymerase with increased affinity at low temperature.

    Katayama S, Matsushita O, Tamai E, Miyata S, Okabe A.

    FEBS Lett. 2001 Dec 7;509(2):235-238.


  1. 36) Mutations that increase the activity of the promoter of the Escherichia coli melibiose operon improve the binding of MelR, a transcription activator triggered by melibiose.

    Tamai E, Belyaeva TA, Busby SJ, Tsuchiya T.

    J. Biol. Chem. 2000 Jun 2;275(22):17058-17063.


  1. 37) Melibiose carrier of Escherichia coli: use of cysteine mutagenesis to identify the amino acids on the hydrophilic face of transmembrane helix 2.

    Matsuzaki S, Weissborn AC, Tamai E, Tsuchiya T, Wilson TH.

   Biochim Biophys Acta. 1999 Aug 20;1420(1-2):63-72.


  1. 38) Conversion of temperature-sensitive to -resistant gene expression due to mutations in the promoter region of the melibiose operon in Escherichia coli.

    Tamai E, Shimamoto T, Tsuda M, Mizushima T, Tsuchiya T.

    J. Biol. Chem. 1998 Jul 3;273(27):16860-16864.


  1. 39)  Purification of UhpT, the sugar phosphate transporter of Escherichia coli.

    Tamai E, Fann MC, Tsuchiya T, Maloney PC.

    Protein Expr. Purif. 1997 Jul;10(2):275-282.